Centre for Integrative Immune Analysis
Our Mission
To enable in-depth immunological analysis for translational and clinical studies.
Our Expertise
Advanced immunological assays and bioinformatics pipelines.
Our Clients
- UHN Investigators
- External Academic Investigators
- Multi-Institute Networks
- Industry
Overview
Specimen Processing
Blood
NaHep | EDTA | Streck | SST
whole blood, buffy coat, PBMCs, plasma, serum
Tissues
Core & Punch Biopsies | Surgical excisions
FFPE, OCT, snap-frozen, single-cells
Fluids
Fluids (ascites, pleural effusions, paracentesis
single-cells, supernatant
*Stool
*kits & storage only
*Saliva
*kits & storage only
Short & Long-term Storage
Short-term: -80°C Long-term: -196°C
Assays & Protocols
Flow Cytometry
(Traditional & Spectral Flow)
T/B/NK, T/Treg, myeloid subsets
CyTOF
(40+ marker panels)
immune phenotyping including checkpoint/activation markers
Cytokines
(1 to 80-plex)
cytokines, chemokines, growth factors
Cell Sorting
(Up to 6 populations)
PBMCs, tumor-infiltrating lymphocytes for scRNA-seq
Genomics
PMGC & OICR-TGL
sample preparation and data analysis: scRNA-seq, CITE-seq, TCR-seq
IHC
(Traditional & Multiplex)
1 to 6 markers on a single slide (FFPE)
Specificity Assays
ELISpot, re-stimulation
IFN-ɣ production, multimer staining
Metabolomics
HPLC, mass-spec (NRC)
IDO activity, immuno-metabolomic profiling
TILs
Expansion in IL-2
TIL profiling
Our Team
Executive Team
Ben Wang
Head, Centre for Integrative Immune Analysis
Marcus Butler
Clinical Head, Centre for Integrative Immune Analysis
Pam Ohashi
Director, TIP Senior Scientist
Naoto Hirano
Immuno-Oncology Chair, PM Senior Scientist
Trevor Pugh
Director of Genomics, OICR Senior Scientist
David Brooks
Senior Scientist
Tracy McGaha
Senior Scientist
Linh Nguyen
Head, Centre for Cell Manufacturing
Hal Berman
Pathologist
Adam Gehring
Senior Scientist
Connect
How to Connect
By email: ben.wang@uhn.ca
When to Connect
Anytime for consultation
During protocol development or prior to an amendment
Preparing a grant submission
Specimens ready for analysis (e.g. PBMCs, FFPE tissues, etc.)
Datasets ready for analysis (e.g. scRNA-seq, CITE-seq, etc.)