Immune Profiling Team

Dr. Marcus Butler with the Immune Monitoring Lab researchers
Back (left to right): Thomas Pfister, Michael Bentley, Valentin Sotov, Diana Gray, Ramy Gadalla, Reema Deol, Drew Wallace, Darya Lemiashkova, Mark Camacho, Diane Liu, Cindy Yang, Douglas Millar, Babak Noamani
Front (left to right): Pamela Ohashi, Naoto Hirano, Marcus Butler, Ben Wang, Tracy McGaha, David Brooks
Not in photo: Andrew Elia, Trevor Pugh


The Immune Profiling Team is led by Dr. Marcus Butler and Dr. Ben Wang. The team works closely with investigators to develop protocols for clinical and pre-clinical studies. The team’s activities range from collection of blood and tissue samples from cancer patients, to processing these samples, to performing a variety of immune monitoring analyses.

The expertise of the Immune Monitoring Team includes the following:

  1. Processing and storage of various blood components (peripheral blood mononuclear cells, serum, plasma).
  2. Processing and viably freezing and storage of tissues (fresh core biopsies and surgical excisions).
  3. Multicolor, high dimensional flow cytometry.
  4. Fluorescence-activated cell sorting (FACS).
  5. Mass cytometry (CyTOF).
  6. Immunohistochemistry (IHC), including multicolor IHC.
  7. Functional assays of various immune cell types.
  8. Analysis of serum or plasma for various proteins, cytokines/chemokines, and or metabolites using methods including ELISAs, multiplex assays and high-performance liquid chromatography (HPLC).
  9. T cell receptor sequencingidentification and repertoire analysis.
  10. Tumor-associated antigen epitope prediction.

The Immune Monitoring Team members are: David Brooks, Marcus Butler, Derek Clouthier, Mark Comacho, Reema Deol, Andrew Elia, Diana Gray, Marie-Jo Halaby, Naoto Hirano, Diane Liu, Douglas Millar, Linh Nguyen, Megan Nelles, Jessica Nie, Tracy McGaha, David Mulder, Pam Ohashi, Michael Pniak, Trevor Pugh, Aashiq Rahman, Michael Ryczko, Valentin Sotov, Cindy Yang, Patty Yen.